ஜர்னல் ஆஃப் கிளைகோபயாலஜி
திறந்த அணுகல்

சுருக்கம்

Pro-angiogenic Activity Assay of Chondroitin Sulfate and Glucosamine Sulfate on Vascular Network of Mouse and of Chick Embryo Chorioallantoic Membrane

Fernanda Katharine de Souza Lins Borba1,2, Edbhergue Ventura Lola Costa1, Viviane Aparecida Balvedi Polli2, Daniela Sousa Coelho2, Marcelo Maraschin2, Paulo Fernando Dias2 and Romildo Albuquerque Nogueira1*

Objective: Target of this study was to test the capacity of chondroitin sulfate (CS) and glucosamine sulfate (GS) to induce in vivo angiogenesis. Methods: The proangiogenic activity of these compounds was analyzed through the assays in chorioallantoic membrane (CAM) of chick embryo and dorsal skin vascularization in mice, but before was realized a cell viability assay with human umbilical veins endothelial cells (HUVEC). Results: In the viability assay, concentrations tested between 30 and 3000 μg/ml showed a reduction of viable HUVEC number. In the CAM assay, CS and GS in an amount 2.0 mg/implant increased the vessels number as compared to control (phosphate buffered saline-PBS). In the assay of the dorsal skin vascularization of adult Swiss mice, the groups treated with CS (2 mg/implant; Gelfoam plug) exhibited an increase in the vessels number into plugs (0.52 ± 0.08 g/dl; measured as plug-hemoglobin content), a similar effect to that promoted by Fibroblast growth factor-2 (FGF-2; 50 ng/implant) (0.53 ± 0.1 g/dl). However the group treated with GS did not exhibit significant effect on mice skin vascularization. Conclusion: CS was capable to promote angiogenesis on CAM and dorsal skin vascularization, but GS only had pro-angiogenic activity in CAM vascular network.