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N-Tetracosanyl Benzoate: A Novel Phytoconstituent of Berberis aristata DCs

Katiyar D, Dixit PK, Singh V, Gilani SJ and Goel R

Numerous investigations have already been done on the roots and stems of Berberis aristata DC. var. aristata (Berberidaceae) and thus the present study involves the isolation and characterization of the ethanolic extract of the heartwood of Berberis aristata. The extraction of the drug (1.8 kg) was carried out in 95% ethanol using Soxhlet apparatus and the extract was concentrated. The slurry was prepared and packed in the column for isolating various phyto constituents using solvents of increasing polarity. The isolated phyto constituents were then characterized and their structures were elucidated using various spectral data analysis i.e., IR, 1HNMR, 13CNMR and +ve ion FAB MS. Elution of the column with chloroform gave the colourless crystals of a novel compound: n-tetracosanyl benzoate having Rf value 0.49 (Benzene: chloroform, 1:1) and m.p. 60°C-61°C.