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Quantification of Antifungal Drug Voriconazole in Serum and Plasma by HPLC-UV

Peter H Tang

A simple, sensitive and rapid high-performance liquid chromatographic (HPLC) method for the determination of voriconazole in human serum or plasma was developed. Voriconazole and internal standard clonazepam were extracted from plasma or serum with methanol and analyzed on a Microsorb-MV C18 column with ultraviolet (UV) detection set at wavelengths of 256 and 310 nm, respectively. The calibration curve was linear through the range of 0.1-10 mg/L using a 0.1 mL sample volume. The within-run and between-run precisions were all less than 6%. Accuracies ranged from 97 to 106%. Absolute recovery was 96.4 ± 1.3% for voriconazole. The method has been applied to monitor voriconazole use in order to ascertain clinical efficacy and minimize toxic effects.