மருத்துவ மற்றும் பரிசோதனை இருதயவியல்
திறந்த அணுகல்

சுருக்கம்

Process of Coronary Capillary Tube Formation by Mononuclear Cells Expressing β-Actin in the Ischemic Myocardium

Yuko Maezawa, Nobuyuki Hiruta, Yasuto Uchida, Yoshiro Maezawa, Toshihiro Morita and Yasumi Uchida

Background: Capillary tube formation is essential for angiogenesis. It was previously thought that capillary tubes are formed through self-division of pre-existing endothelial cells (ECs) or folding of EC-progenitor cells. We found that circulating mononuclear cells that express β-actin (β-MNCs), participate in arteriogenesis, (formation of arterioles and arteries, and their growth) but it remained obscure whether and how they participate in capillary tube formation.
Methods: We induced acute myocardial infarction in beagles, and examined the process of capillary tube formation by β-MNCs in the infarcted myocardium.
Results: β-MNCs were recruited to coronary vessels, in particular to capillaries, in the border zone of infarcted myocardium. β-MNC nuclei were either round or flat, indicating existence of several species of β-MNCs. They sprouted out from a pre-existing coronary arteriole, capillary or venule into the interstitial space, and adhered to each other in tandem. An intracellular cavity was formed by the loss of granular cytoplasm in each cell. The intercellular junctions which connected cell-to-cell disappeared, and the cavities connected with each other to form a capillary tube. New β-MNCs moved to the distal end of the tube and migrated outside to extend the capillary tube. ECs of the capillary tubes thus formed possessed CD₃₁, a marker for EC, but not β-actin. Their nuclei were either round or flat, indicating existence of two different capillary tubes.
Conclusions: Coronary capillary tube formation in the ischemic myocardium is initiated by β-MNCs, not by their folding but by intracellular cavity formation through the loss of their granular cytoplasm and consequent tube formation by cavity-to-cavity connection.