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In- vitro analysis of antibacterial properties of fresh and dry waste peels of fruits and vegetables against gram positive and gram negative pathogens

Shivangi Khare

Phytochemicals present in all parts of plants are of great significance to humans in various ways, they contribute life sustaining properties and have a potential to provide vigor against diseases viz. cancer and cardiovascular diseases. The objective of this analysis was to acquire enough amounts of data about the anti-bacterial activity of fresh and dry form waste of fruits and vegetables against three different pathogens viz. Escherichia coli, Klebsella pneumoniae, Bacillus subtilis.
The extracts were prepared via solvent extraction method by dissolving different samples of waste fruits and vegetables in solvents viz Hexane, Petroleum ether, Methanol and Acetone 48 hours, these solvents were kept under dark condition for two days so that the solvent retains its efficiency. Samples were then subjected to Anti- microbial susceptibility test and the analysis revealed that fresh Acetone and Dry Methanol extract of Precap of Punica granatum gave the best result against Escherichia coli, Klebsella pneumoniae, Bacillus subtilis with zone of inhibition 20 mm, 23 mm, & 20 mm and 18 mm, 22mm & 18.5. Similarly almost all extracts of Dry and Fresh B. oleracea var capitata, B. oleracea var botrytis, A. sativum, A. fistulosum, P. sativum, & C. arietinum also gave result against three pathogens. With metal ion Mg++, Fe+++ and pH (5, 7, 9, 11) try to enhance the activity of all extracts but their activity not enhanced it will be suppressed. Minimal Inhibitory concentration of Methanol extract of dry Punica granatum precap against K. pneumonia is 39.06 mg/ml and Acetone extract of Acetone Punica granatum precap against K. pneumonia is 0.238 mg/ml.