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Hepatoprotective Efficacy of Gold Nanoparticle Synthesized by Green Method Using Trigonella Foenum-Graecum Seed Extract

Sourav Ghosh, Antony Gomes*

Earlier study showed the effective hematopoietic potential of gold nanoparticle (FG-GNP) synthesized using fenugreek seed aqueous extract. The present work was an effort to establish the hepatoprotective activity of FG-GNP in animal model. Swiss albino male mice (n=4) were grouped into: Gr.1- Sham Control, Gr.2- CCl4 treated (1.5ml/kg), Gr.3- Standard Drug (Silymarin) treated (100mg/kg/p.o.), Gr.4- Fenugreek treated (100mg/kg/p.o.), Gr.5- FG-GNP treated (0.5mg/kg/p.o.). Biochemical parameters (AST, ALT, γGT, ACP, ALP, total bilirubin), pro-inflammatory parameters (IL 1β, IL 17, TNF α), anti-inflammatory parameters (IL 10, Cathepsin K), antioxidant parameters (GSH, SOD, Catalase) and prooxidant parameter (LPO) were assayed. Histology of liver tissue was done. AST and ALT release from liver tissue was done by in vitro liver slice model. Statistical analysis was done by one way ANOVA, values expressed as mean±SE, P<0.05 was considered as statistically significant. CCl4 induction caused significant increased the serum biochemical parameters, pro-inflammatory parameters, prooxidant and decrease in anti-inflammatory parameters and anti-oxidants in animal model and increase in AST, ALT release in in vitro model. Treatment with FG-GNP caused significant decrease in biochemical parameters, pro-inflammatory parameters, prooxidant and increase in anti-inflammatory parameters, anti-oxidants in animal model and decrease in AST, ALT release in in vitro model when compared to hepatotoxic control animals. FG-GNP partially recovered CCl4-induced damages observed in liver histology. The present study showed hepatoprotective potential of FG-GNP which was >FG. Further studies regarding molecular actions of FG-GNP are warranted.