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In Vitro Study on Tribologically Micronized Zeolite as A Natural Cell Protecting Chelator of Lead (II) Acetate

Tugba Sagir*, Mehmet Kenar, Gokhan Ozokan, Kerim Bilginer

In order to determine whether clinoptilolite, a naturally occurring zeolite, had any toxic effect on keratinocyte cells when combined with lead (II) acetate, real time cell analysis and MTS assays were performed. Herein, Micronized Zeolite (MZ) was obtained by tribomechanical micronization of natural clinoptilolite. The effect of lead (II) acetate (Pb (CH₃COO) ₂.3H₂O) adsorption of clinoptilolite at different durations (30 minutes, 1 hour, 2 hours, 4 hours, 8 hours) was investigated. When 5% clinoptilolite was applied to the lead (II) acetate solution, the adsorption capacity was 55.15% in 30 minutes, 58.09% in 1 hour, 71.62% in 2 hours, 82.66% in 4 hours, 87% in 8 hours. These results were in parallel with cell culture studies which showed when lead is treated with tribologically micronized zeolite (Clinoptilolite) it increases the cell viability.

Clinoptilolite, had an adsorbing effect on lead (II) acetate, did not show toxic effect on keratinocyte cells, and even eliminated lead (II) acetate toxicity. It might suggest an alternative natural cell protecting chelator.