ஐ.எஸ்.எஸ்.என்: 2471-9552
Jie Cao, Zexin Guo, Wei Zhou, Yueqing Wu, Lixia Dong, Jing Feng , Shuo Li
Objective: To elucidate potential IL-17A- and TSA-mediated regulation of fibroblasts transformation.
Method: MTT assay, HDAC1 activity assay, cell immunofluorescence and Western blot were employed to detect the expression of related indicators.
Conclusion: IL-17A stimulates proliferation of MRC5 cells and increases HDAC1 activity and protein expression. It also transforms MRC5 cells into myofibroblasts via activation of the TGF-β1/Smads signaling network. TSA, on the other hand, strongly suppresses TGF -β1/Smads pathway-mediated fibrosis by ceasing HDAC1 activity and protein expression.