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Role of Six Trans Membrane Protein of Prostate (STAMP) Proteins in Prostate Cancer- Relation with Survival Genes

Ceren Gonen-Korkmaz, Gulnur Sevin, Goksel Gokce, Mehmet Zuhuri Arun, Gunay Yetik- Anacak, Gokce Yıldırım, Lokman Varisli, Buket Reel, Aysegul Kaymak, Mazen Saeed Abdulaziz and Deniz Ogut

Prostate cancer studies focus on identification of androgen receptor (AR) regulated genes that are also highly expressed in the prostate. As a promising candidate, STAMP family genes STAMP1/STEAP2, STAMP2/STEAP4 and STEAP3 are involved in apoptosisand the cell cycle in metastatic prostate cancer. Vascular NADPH oxidase generates superoxide and other ROS, which stimulates IkappaB degradation and NF-kB activation by subunits of NADPH oxidases, namely p47phox and p67phox induced by different stimuli such as hydrogen peroxide. Hydrogen peroxide increased the expression levels of p67phox. They also have a role in redox-sensitive genes such as STAMP gene family. Flow cytometry analysis of LNCaP cells was performed using Annexin V staining and apoptotic index charts were drawn. STAMP1 and STAMP2 showed total anti-oxidant capacity versus control with hydrogen peroxide incubation. Using siRNA technology in LNCaP cells expressing mutant p53 silencing of p53 showed significant increase in MDM2 and decrease of caspase 9 mRNA levels at RT-PCR. Silencing of STAMP2, a significant decrease in p47phox was shown but STAMP1 silencing counteracted this effect on Cu/ZnSOD expression.As a conclusion, STAMP proteins have effects on oxidative stress-induced genes with significant and opposite changes.