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Occurrence and Detection of Chimeral Sectors in Leaf Tissue of Novel Lolium multiflorum x L. arundinaceum Hybrids

Bryan Kindiger

A novel diploid, Lolium multiflorum (Lolium perenne L. subsp. multiflorum (Lam.) Husnot (syn. Lolium multiflorum Lam.) line has been previously observed to induce genome loss and dihaploid generation following hybridization with hexaploid, L. arundinaceum (Schreb.) Darbysh.) (Syn.Festuca arundinacea Schreb.). Early observation of chimeral sectors in these F1 has suggested a form of mitotic chromosome or genome loss was responsible for the recovery of the L. multiflorum or L. arundinaceum dihaploids, and that flow cytometry was a methodology that can be utilized to observe such events. Evaluations of leaf tissue nuclei, extracted from 21 F1 hybrids, were submitted to flow cytometry analysis to obtain ploidy estimation within those leaf tissue sections. Results of the analysis suggest a high degree of mitotic genome instability in the leaf tissue sections, providing indicated that mitotic chromosome or genome instability was one cause for dihaploid generation in these materials. A comparison regarding the frequency of sectoring across the various L. arundinaceum genotypes, as well as a comparison of greenhouse vs field nursery growing conditions, did not indicate any positive or negative influences.