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Jatropha Curcas Linn can Reduce the Expression of Hsp70 that will Result in Reduced Errors in Protein Folding and Promotion of Normal Protein Function in Proliferation and Apoptosis

Prayitno A, Fitria MS, Elmanda AY and Astirin OP

Background: Jatropha curcas (J. curcas) as a local plant have phytochemical contents like phenolic that effect as anti-inflammatory and cytotoxicity properties. Research in the last decade about cancers showed that over expression of heat shock protein 70 (Hsp70) does to mis-folding and continues to triggering proliferation and suppressing apoptosis. Increased expression of Hsp70 is responsible for mis-folding of proteins and implicated in functions that lead and play a role in the pathogenesis of pain including cancer incidence. In this study, we examined the expression of Hsp70, pRb and caspase3 in Raji cells after treatment with J. curcas.

Methods: We first to determinethe plant. The plant is shrubby, woody and many are found in the tropics, names J.curcas sp. (Euphorbiaceae family). Second, to extraction of fresh leaves taken from J. curcas, washed clean, dried by the wind, sliced into small pieces and end dried by an oven at a temperature 65°C for 48 hours. And third, To developed a Raji cell culture (a cancer model) system and treated cells with fractionated extracts of J. curcas leaf. Fourth are evaluating the expression of Hsp70, pRb and caspase-3 that do by immuno histochemical analysis. A semi quantitative (devided into catagories: low, medium and strong) data was collected under light microscope view.

Results: Our results showed that the expression of Hsp70 in Raji cells after treatment with fractionation J. curcas leaf was strong as much as 9(30.00%) lower than control (14:46.60%). The expression of pRb after treatment with fractionation J. curcas leaf was strong as much as 15(50.00%) higher than control (9:30.00%). And the expression of caspase-3 in Raji cells after treatment with fractionation J. curcas leaf was strong as much as 13(43.3%) higher than control (9:30.00%).

Conclusion: Our results show that J. curcas leaf can reduce the expression of Hsp70, suggesting it can also reduce errors in protein folding and promotion of normal protein function in proliferation (by inhibit pRb protein expression) and apoptosis (by enhance caspase-3 protein expression).