select ad.sno,ad.journal,ad.title,ad.author_names,ad.abstract,ad.abstractlink,j.j_name,vi.* from articles_data ad left join journals j on j.journal=ad.journal left join vol_issues vi on vi.issue_id_en=ad.issue_id where ad.sno_en='103189' and ad.lang_id='10' and j.lang_id='10' and vi.lang_id='10' Efficient Production of High Resistant Starch Rice through T | 103189
என்சைம் பொறியியல்

என்சைம் பொறியியல்
திறந்த அணுகல்

ஐ.எஸ்.எஸ்.என்: 2329-6674

சுருக்கம்

Efficient Production of High Resistant Starch Rice through Targeted Mutagenesis of Starch Branching Enzyme Ⅱb By CRISPR/Cas9

Hsi-Chao Wang, Su-Ying Yeh, Yong-Pei Wu, Yu-Chia Hsu, Maurice S. B. Ku*

Rice is the staple food for half of the world’s population. Rice starch is low in Resistant Starch (RS) with a high
Glycemic Index (GI). RS has gained importance since it is beneficial in preventing various diseases. Starch Branching
Enzyme IIb (SBEIIb) plays a key role in amylopectin synthesis in the endosperm of cereals. In this study, we mutated
OsSBEIIb in the Japonica rice cultivar Tainung82 (TNG82) through CRISPR/Cas9 and investigated the molecular and
physicochemical modifications in OsSBEIIb mutant lines, e.g., gene expression, enzyme activity, Amylose Content
(AC), RS and GI. As expected, gene expression and enzyme activity of OsSBEIIb were down-regulated significantly
while AC and RS contents increased progressively from 17.4% and 0.5% in weight, respectively, to as high as
25.0% and 7.5% in heterozygous mutant lines and 36.0% and 12.0% in homozygous mutant lines. Consequently,
with increased RS and decreased rate of reducing sugar production, GI progressively decreased in heterozygous
and homozygous mutant rice endosperms by 11% and 28%, respectively. Transgene-free plants were subsequently
identified in the T1 populations. Our results demonstrate the precise and efficient generation of high RS and low
GI transgene-free rice through CRISPR/Cas9 to provide a more suitable source of starch for type II diabetes.

மறுப்பு: இந்த சுருக்கமானது செயற்கை நுண்ணறிவு கருவிகளைப் பயன்படுத்தி மொழிபெயர்க்கப்பட்டது மற்றும் இன்னும் மதிப்பாய்வு செய்யப்படவில்லை அல்லது சரிபார்க்கப்படவில்லை.
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