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Comparison of Connective Tissue Growth Factor Expression in Urethral Stricture Patients due to Infection and Trauma with IHC and ELISA Methods

Safendra Siregar, Nanda Daniswara, Jupiter Sibarani, Tjahjodjati T, Kuncoro Adi, Hernowo Bethy, Anglita Yantisetiasti, Ris Kristiana, Anna Tjandrawati

Objectives: We look for the difference of CTGF expression in urethral stricture due to infection and trauma with Immunohistochemistry (IHC) and Enzyme- linked Immunosorbent Assay (ELISA) methods.
Methods: We measure the CTGF level from 5 urethral stricture tissue of patient with infection, 18 patients with traumatic urethral stricture. We used IHC and ELISA as method of CTGF measurement. We also compared the result between IHC and ELISA in our subjects. Statistical analysis was done using SPSS version 22.0.
Results: From ELISA measurement, the CTGF level of traumatic urethral stricture patients was significantly higher than patients with infection (5.294 ± 7.213 pg/dl vs. 2.62 ± 2.156 pg/dl, p=0.002). From IHC examination CTGF expression in trauma group was significantly stronger than infection group (p=0.041). There was no significant difference between the result of ELISA examination and IHC examination in measuring CTGF level in urethral infection stricture tissue (p=1.000) and trauma stricture tissue (p=0.112).
Conclusions: CTGF expression is higher in traumatic urethral stricture patients compared to urethral stricture patients due to infection. There were no significant difference between the result of both ELISA and IHC.