Dharmar Manimaran, Vasan Palanisamy*
Peptide Loaded Chitosan Coated (PLCC) and Sodium Alginate (SA) nanoparticles are considered as an effective deliver therapeutic molecules to the target site. PLCC and SA nanoparticles were synthesized by the ionic gelation process and their peptide polymer compatibilities were analysed by Fourier Transform-Infrared Spectroscopy (FT-IR) and X-Ray Diffraction (XRD). Nanoparticles (NPs) morphology Scanning Electronic Microscope (SEM), process yield, association and loading efficiency, in vitro peptide release, size distribution and zeta potentials, kinetic modelling, haemocompatibility, plasma stability, genotoxicity and embryo toxicity studies were performed by using Danio rerio model. High peptide polymer compatibility was high in PLCC than the SA nanoparticles. X-ray diffraction proved that the peptide had been loaded perfectly in the chitosan nanoparticles. SEM analysis showed that PLCC were irregularly arranged circular in shape. Process yield was 19.97%, association efficiency was 83.45%, loading efficiency was 1.85%, release rate was 71.95%, equally distributed and zeta potential was 32.6 ± 4.65. The toxicity screening of the peptide loaded chitosan coated was found to be concentration and time dependent manner.