மருத்துவ மற்றும் நறுமண தாவரங்கள்
திறந்த அணுகல்
ஐ.எஸ்.எஸ்.என்: 2167-0412
ஐ.எஸ்.எஸ்.என்: 2167-0412
Irfan A Ghazi*, Job O Mapesa*, Jessie R Wilburn*, Jan E Leach*, Sangeeta Rao*, Corey D Broeckling*, Anna McClung*, Elizabeth P Ryan and Iman Zarei*
Dietary rice bran supplementation has been shown to inhibit Salmonella fecal shedding in animals. The aim of this study was to determine if bran extracts from two distinct rice varieties, Lijiangxintuanheigu (LTH)d and Sanhuangzhan-2 (SHZ-2), differentially inhibit Salmonella enterica serovar Typhimurium invasion and intracellular replication. Rice bran extracts were tested in vitro using mouse small intestine epithelial (MSIE) and intestinal porcine epithelial cells (IPEC-J2). Fluorescent labeled Salmonella was detected using fluorescence microscopy and culture based methods. Non-targeted metabolomics using ultra-performance liquid chromatography- mass spectrometry (UPLC-MS) was performed on LTH and SHZ-2 rice bran extracts. LTH bran extract dose-dependently reduced entry and intracellular replication of Salmonella in both MSIE and IPEC-J2 cells when compared with SHZ-2. The rice bran metabolite profiling revealed significant variations between LTH and SHZ-2. LTH had higher total numbers of metabolites (429) versus SHZ-2 (407), with increased relative abundance of lipids (i.e., galactolipids and phospholipids) and flavonoids compared with SHZ-2. SHZ-2 had higher levels of dipeptides and phenylpropanoids. Distinct metabolite differences between LTH and SHZ-2 revealed rice bran components that may be responsible for blocking Salmonella invasion and intracellular replication. Metabolomics is a powerful phenotyping tool for identifying rice bran compounds with protective effects against pathogens. Future studies may identify the rice genes responsible for these bioactive rice bran metabolites distinguishing LTH from SHZ-2, and enable genetic selection for compounds as traits that offer important health and disease fighting benefits.