ஜர்னல் ஆஃப் நானோமெடிசின் & பயோதெரபியூடிக் டிஸ்கவரி
திறந்த அணுகல்

சுருக்கம்

Nanomedicine & Drug Delivery : New biocompatible oxide nanoparticles as carriers of bioactive compounds through the blood-brain barrier Michal M Godlewski- Warsaw University of Life Sciences

Michal M Godlewski

Blood-brain barrier is major obstacle for drug delivery to the brain. In this study, we focused on oxide Nano Particles (NPs) as potential drug carriers. Mice received suspension of Y2O3:Tb:Lectin NPs (10 mg/ml; 0.3 ml/mouse) via gastric gavage (IG) and were sacrificed after 24 hr, 48 hr and 1 week. Control group received equivalent suspension of pure lectin. All protocols were conducted according to EU guidelines and approved by LEC agreement no. 44/2012. Following the sacrifice, brain tissue was collected for the analyses under confocal microscope and scanning cytometry. Lectins were chosen as a perfect model substance for the use of NPs as carriers, since physiologically they are not absorbed from the gastrointestinal tract. Control group exhibited extremely low signal for lectin not exceeding background level. In the group which received Y2O3:Tb:Lectin, signal for lectin coincided with NPs red fluorescence in the brain as soon as 24 hr after IG. Following 48 hours, the convergence lowered and after 1- week only free lectin was observed in the brain tissue. In conclusion, oxide NPs proved able to transport bioactive compounds through the blood-brain barrier. After entering brain tissue complexes of nanoparticles and lectin dissolved and free lectin was deposited in the tissue.