ஜர்னல் ஆஃப் கிளினிக்கல் & எக்ஸ்பெரிமென்டல் டெர்மட்டாலஜி ரிசர்ச்
திறந்த அணுகல்

சுருக்கம்

Molecular Characterization of the Skin Fungal Microbiota in Patients with Seborrheic Dermatitis

Akiomi Tanaka, Otomi Cho, Mami Saito, Ryoji Tsuboi, Sanae Kurakado and Takashi Sugita

Background: Seborrheic dermatitis (SD) is an inflammatory disease associated with seborrhea that appears most often on the face, especially the nasolabial folds, scalp, and upper trunk, in sebaceous gland-rich areas of the skin. Although various factors are involved in the development of SD, Malassezia species of skin fungi play an important role in this skin disease. The aim of this study was to obtain basic information to elucidate the mycological involvement in the development of SD by performing a comprehensive analysis of the skin fungal microbiota of lesional and non-lesional sites in SD patients using a pyrosequencing approach.

Methods: Scale samples were collected from lesional and non-lesional sites of 24 Japanese patients with seborrheic dermatitis and the skin fungal microbiome in the scale samples was analyzed using a pyrosequencing method.

Results: Thirty taxa were detected from a total of 480,186 high-quality sequences. Malassezia species predominated at both lesional and non-lesional sites; however, the fungal microbiota at lesional sites was more diverse than at non-lesional sites. The fungal communities at lesional and non-lesional sites were clearly distinguished on principal coordinate analysis.

Conclusion: Our data indicated that the skin fungal microbiota in lesional sites of SD patients was independent of that in non-lesional sites and will be utilized as basic information for understanding the association between skin fungi and development of SD.