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In vitro Propagation of Kainth (Pyrus pashia) Using Explants from Forced Cutting

Rehman HU

This study was carried out in Tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M1), MS (M2) and WPM (M3)} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Maximum establishment (63.60%) was obtained on M2 containing BAP (1.5 mgl-1) and IBA (0.25 mgl-1). Maximum proliferated cultures (95.30%) and shoots per explant were obtained using M3 medium fortified with BAP (3.0 mgl-1). However, shoots of maximum length (42.97 mm) were obtained in M3 medium containing BAP (0.0 mgl-1) i.e control. Rooting (%), roots per explant and root length was found to be influenced by type of medium and growth regulator fortification. Rooting (%) was maximum (13.34%) was observed in M1 medium supplemented with IBA (0.1 mgl-1). However, NAA (1.0 mgl-1) induced rooting of 29.61 per cent using M1 medium. Maximum roots per explant were obtained using M1 medium supplemented with IBA (1.0 mgl-1). NAA (1.0 mgl-1) induced highest roots per explant i.e 3.40 using M1 medium. However, M3 supplemented with IBA (0.1 mgl-1) resulted in maximum root length of 31.15 mm. NAA (0.1 mgl-1) resulted in maximum root length of 22.97 mm using M1 medium.