select ad.sno,ad.journal,ad.title,ad.author_names,ad.abstract,ad.abstractlink,j.j_name,vi.* from articles_data ad left join journals j on j.journal=ad.journal left join vol_issues vi on vi.issue_id_en=ad.issue_id where ad.sno_en='57931' and ad.lang_id='10' and j.lang_id='10' and vi.lang_id='10' Effect of Stemregenin1 and SB431542 small molecules on ex vi | 57931
ஜர்னல் ஆஃப் பெரியோபரேட்டிவ் மெடிசின்

ஜர்னல் ஆஃப் பெரியோபரேட்டிவ் மெடிசின்
திறந்த அணுகல்

ஐ.எஸ்.எஸ்.என்: 2684-1290

சுருக்கம்

Effect of Stemregenin1 and SB431542 small molecules on ex vivo expansion of umbilical cord blood hematopoietic stem cells on biocompatible polyether sulfone nanofiber scaffolds

Sorush Niknamian

Cord blood hematopoietic stem cells (HSCs) with several advantages including low chance of viral contamination and low rate of Graft versus host disease (GvHD) are appropriate candidate for vast medical applications such as transplantation. The main obstacle of cord blood HSCs is the low number cells. To improve ex vivo expansion of umbilical cord HSCs author introduced a new culture system. Isolated HSCs were seeded in three-dimensional (3D) on Polyethersulfone (PES) scaffolds and two-dimensional (2D) culture conditions and treated with SB431542 and Stemregenin1 (SR1) small molecules. On the fifth and tenth days the expanded cells in different groups were investigated for expression of specific markers by flow cytometry, expression of some stemness genes by qRT-PCR and colony formation by methocult medium. SR1 molecule significantly increased expansion of CD34+ cells while SB431542 induced more CD34+/38+ cells. Also SB431542 treated cells showed higher colony formation capacity. SR1 increased the expression of c-Myc, HOXB4 and SALL4 while SB431542 seemed to inhibit HOXB4 expression and increase SALL4.Together this study introduced a new ex vivo culture setting for further medical application of HSCs. Their data showed simultaneous use of these two small molecules can provide appropriate outcome for HSCs transplantation includes both of engraftment and repopulation.

மறுப்பு: இந்த சுருக்கமானது செயற்கை நுண்ணறிவு கருவிகளைப் பயன்படுத்தி மொழிபெயர்க்கப்பட்டது மற்றும் இன்னும் மதிப்பாய்வு செய்யப்படவில்லை அல்லது சரிபார்க்கப்படவில்லை.
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