எலும்பு ஆராய்ச்சி இதழ்
திறந்த அணுகல்

சுருக்கம்

ஆஸ்டியோபோரோசிஸ் உள்ள மற்றும் இல்லாத இந்திய மாதவிடாய் நின்ற பெண்களின் ஆஸ்டியோக்ளாஸ்ட் முன்னோடி செல்களில் வேறுபட்ட மரபணு வெளிப்பாடு முறை: ஒரு மைக்ரோஅரே அடிப்படையிலான ஆய்வு

மெஹ்ருன்னிசா எம் ராஜே, சுஹாஸ் டி மஸ்கே, பாயல் கோஷ், மோகன் ஆர் வானி மற்றும் ரிச்சா ஆஷ்மா

Background: Osteoporosis is a multifactorial disease with strong genetic and epigenetic component. In spite of enormous candidate gene association studies, the etiology and molecular mechanism of disease is not fully known. For identification of new markers of osteoporosis which could be vital in diagnosis and prognosis of disease, genome-wide microarray expression approach was employed.
Methods: Osteoclast precursor cells were sorted from circulating monocytes of osteoporotic and nonosteoporotic post menopausal females with similar life-style and year after menopause. Following microarray experimentation, gene enrichment analysis was performed on significant DEGs using Database for Annotation, Visualization and Integrated Discovery (DAVID) tool. Top 10 novel genes were further used for construction of protein-protein interaction network using Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database. To validate microarray gene expression pattern, Real time-PCR was performed.
Results: A total of 269 genes were found to be differentially expressed between disease and normal groups, of which 138 were observed to be up regulated and 131 down regulated. Furthermore, novel LHX1 gene was observed to be up regulated and its interaction with BMP4 protein was observed. Three known genes for osteoclastogenesis (viz., CX3CL1, ACP5 and CSF1) were found to be up- regulated. Similar pattern of gene expression have been obtained using RT-PCR.
Conclusion: Significant enrichment of PI3K-Akt and TGF-ß signaling pathways involving DEGs was found in postmenopausal Asian Indian women. Moreover, up regulated LHX1 gene was discovered as novel gene which may have a role in pathogenesis of osteoporosis and can be used for diagnosis and prognosis along with known osteoporotic markers.