Mengqi Zhang, Xinchen Wu*, Wenning Yang*, Rui Yang
Stable heavy isotopes of bio-elements have potential biological activities which is an issue being neglected. The enrichment of heavy isotope in the molecule of a substance may alter the substance’s effects on organism. In our study, the enrichment level of heavy carbon isotope (13C) in testosterone reached to the isotopic ratio (13C/12C) of 9.78%, by which heavy carbon testosterone was produced. The human cancer cells of breast (MCF-7), ovarian (SK-OV-3), and endometrial (Ishikawa) were exposed to testosterone and heavy carbon testosterone (named 13C enriched testosterone) respectively. The growth and morphology of the cells were studied. The dose impact of the compound was investigated as well. To the best of our knowledge, this is the first study demonstrating the influence of heavy carbon isotope on cancer cells. The enrichment of heavy carbon isotope reduced growth of the cells. The cell morphology did not change significantly.