உணவு இதழ்: நுண்ணுயிரியல், பாதுகாப்பு மற்றும் சுகாதாரம்
திறந்த அணுகல்

சுருக்கம்

Guobiao தரநிலைகள் (GB) முறையுடன் ஒப்பிடும்போது, ​​லூப்-மெடியேட்டட் ஐசோதெர்மல் ஆம்ப்ளிஃபிகேஷன் (LAMP)-Bioluminescent Assays ஐப் பயன்படுத்தி பொடி செய்யப்பட்ட குழந்தை ஃபார்முலா மற்றும் தொடர்புடைய மெட்ரிஸில் குரோனோபாக்டர் மற்றும் சால்மோனெல்லாவை விரைவாகக் கண்டறிதல் .

Yan Huang, Raj Rajagopal*, Jianwei Huo, Zhiyong Dai, Can Yi, Jun Zhou, Chenyan Niu, Chenyan Niu , Feng Liu, Qing Tao , Xiqing Wang.

Cronobacter and Salmonella have emerged as major pathogens of concern in Powdered Infant Formula (PIF). China’s PIF industry is growing, and consumers are concerned about the potential risk of these pathogens in PIF. The PIF producers need rapid, easy to use and specific detection of Cronobacter and Salmonella for monitoring of raw materials, process environment and finished products for implementing effective control measures to prevent contamination. The objective of this study was to determine the specificity and sensitivity of LAMP assays compared to the Chinese National Food Safety (Guobiao, GB) Standards 4789.4-2016 (Salmonella) and GB 4789.40-2016 (Cronobacter) detection in raw materials (milk powder, whey powder, minerals, lutein, nucleotide, arachidonic acid powder, docasahexaenoic acid), process environment samples and finished products (neonate and infant PIF). The respective LAMP assays detected Cronobacter and Salmonella in artificially contaminated samples and the results of the LAMP assays were comparable to the respective GB method. The Probability of Detection (POD) analysis showed no significant differences (95% confidence interval) among all the samples tested for both LAMP assays and the respective GB method. The respective LAMP assays enabled rapid detection of Cronobacter and Salmonella in PIF matrices providing next-day results as compared to 3 to 5 days for the GB method.